Fluorochromes – Excitation and Emission Wavelengths

Below is a very useful table of various Fluorochromes that can be used in research requiring precise responses.? For more information about Fluorescent Microspheres that utilize fluorochromes check out this related article. “Fluorescent Microspheres – Properties and Applications

The fluorochrome table is also available for download as a pdf.

Table of Excitation and Emission WavelengthsFLUOROCHROME EXCITATION(nm)
EMISSION (nm)
3-Hydroxypyrene 5,8,10-Tri Sulfonic acid 403 513
5-Hydroxy Tryptamine 380-415 520-530
5-Hydroxy Tryptamine (5-HT) 400 530
Acid Fuchsin 540 630
Acridine Orange (bound to DNA) 502 526
Acridine Red 455-600 560-680
Acridine Yellow 470 550
Acriflavin 436 520
AFA (Acriflavin Feulgen SITSA) 355-425 460
Alizarin Complexon 530-560 580
Alizarin Red 530-560 580
Allophycocyanin 650 661
ACMA 430 474
Aminoactinomycin D 555 655
Aminocoumarin 350 445
Anthroyl Stearate 361-381 446
Astrazon Brilliant Red 4G 500 585
Astrazon Orange R 470 540
Astrazon Red 6B 520 595
Astrazon Yellow 7 GLL 450 480
Atabrine 436 490
Auramine 460 550
Aurophosphine 450-490 515
Aurophosphine G 450 580
BAO 9 (Bisaminophenyloxadiazole) 365 395
BCECF 505 530
Berberine Sulphate 430 550
Bisbenzamide 360 600-610
BOBO 1 462 481
Blancophor FFG Solution 390 470
Blancophor SV 370 435
Bodipy Fl 503 512
BOPRO 1 462 481
Brilliant Sulphoflavin FF 430 520
Calcien Blue 370 435
Calcium Green 505 532
Calcofluor RW Solution 370 440
Calcofluor White 440 500-520
Calcophor White ABT Solution 380 475
Calcophor White Standard Solution 365 435
Cascade Blue 400 425
Catecholamine 410 470
Chinacrine 450-490 515
Coriphosphine O 460 575
Coumarin-Phalloidin 387 470
CY3.1 8 554 568
CY5.1 8 649 666
CY7 710 805
Dans (1-Dimethyl Amino Naphaline 5 Sulphonic Acid) 340 525
Dansa (Diamino Naphtyl Sulphonic Acid) 340-380 430
Dansyl NH-CH3 in water 340 578
DAPI 350 470
Diamino Phenyl Oxydiazole (DAO) 280 460
Dimethylamino-5-Sulphonic acid 310-370 520
Diphenyl Brilliant Flavine 7GFF 430 520
Dopamine 340 490-520
Eosin 525 545
Erythrosin ITC 530 558
Ethidium Bromide 510 595
Euchrysin 430 540
FIF (Formaldehyde Induced Fluorescence) 405 435
Flazo Orange 375-530 612
Fluorescein Isothiocyanate (FITC) 490 525
Fluo 3 485 503
Fura-2 340-380 512
Genacryl Brilliant Red B 520 590
Genacryl Brilliant Yellow 10GF 430 485
Genacryl Pink 3G 470 583
Genacryl Yellow 5GF 430 475
Gloxalic Acid 405 460
Granular Blue 355 425
Haematoporphyrin 530-560 580
Hoechst 33258 (bound to DNA) 346 460
Indo-1 350 405-482
Intrawhite Cf Liquid 360 430
Leucophor PAF 370 430
Leucophor SF 380 465
Leucophor WS 395 465
Lissamine Rhodamine B200 (RD200) 575 595
Lucifer Yellow CH 425 528
Lucifer Yellow VS 430 535
Magdala Red 524 600
Maxilon Brilliant Flavin 10 GFF 450 495
Maxilon Brilliant Flavin 8 GFF 460 495
MPS (Methyl Green Pyronine Stilbene) 364 395
Mithramycin 450 570
NBD Amine 450 530
Nile Red 515-530 525-605
Nitrobenzoxadidole 460-470 510-650
Noradrenaline 340 490-520
Nuclear Fast Red 289-530 580
Nuclear Yellow 365 495
Nylosan Brilliant Flavin E8G 460 510
Pararosaniline (Feulgen) 570 625
Phorwite AR Solution 360 430
Phorwite BKL 370 430
Phorwite Rev 380 430
Phorwite RPA 375 430
Phosphine 3R 465 565
Phycoerythrin R 480-565 578
Pontochrome Blue Black 535-553 605
Primuline 410 550
Procion Yellow 470 600
Propidium Iodide 536 617
Pyronine 410 540
Pyronine B 540-590 560-650
Pyrozal Brilliant Flavin 7GF 365 495
Quinacrine Mustard 423 503
Rhodamine 123 511 534
Rhodamine 5 GLD 470 565
Rhodamine 6G 526 555
Rhodamine B 540 625
Rhodamine B 200 523-557 595
Rhodamine B Extra 550 605
Rhodamine BB 540 580
Rhodamine BG 540 572
Rhodamine WT 530 555
Rose Bengal 540 550-600
Serotonin 365 520-540
Sevron Brilliant Red 2B 520 595
Sevron Brilliant Red 4G 500 583
Sevron Brilliant Red B 530 590
Sevron Orange 440 530
Sevron Yellow L 430 490
SITS (Primuline) 395-425 450
SITS (Stilbene Isothiosulphonic acid) 365 460
Stilbene 335 440
Snarf 1 563 639
sulphO Rhodamine B Can C 520 595
Sulpho Rhodamine G Extra 470 570
Tetracycline 390 560
TRITC (Tetramethyl Rhodamine Isothiocyanate) 557 576
Texas Red 596 615
Thiazine Red R 510 580
Thioflavin S 430 550
Thioflavin TCN 350 460
Thioflavin 5 430 550
Thiolyte 370-385 477-484
Thiozol Orange 453 480
Tinopol CBS 390 4.3
TOTO 1 514 533
TOTO 3 642 661
True Blue 365 420-430
Ultralite 656 678
Uranine B 420 520
Uvitex SFC 365 435
Xylene Orange 546 580
XRITC 582 601
YO PRO 1 491 509

Fluorescent Microspheres ? Properties and Applications

Share with colleagues: