Everything about Microspheres
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  • Fluorochromes – Excitation and Emission Wavelengths

    Below is a very useful table of various Fluorochromes that can be used in research requiring precise responses.  For more information about Fluorescent Microspheres that utilize fluorochromes check out this related article. “Fluorescent Microspheres – Properties and Applications

    The fluorochrome table is also available for download as a pdf.

    Table of Excitation and Emission WavelengthsFLUOROCHROME EXCITATION(nm)
    EMISSION (nm)
    3-Hydroxypyrene 5,8,10-Tri Sulfonic acid 403 513
    5-Hydroxy Tryptamine 380-415 520-530
    5-Hydroxy Tryptamine (5-HT) 400 530
    Acid Fuchsin 540 630
    Acridine Orange (bound to DNA) 502 526
    Acridine Red 455-600 560-680
    Acridine Yellow 470 550
    Acriflavin 436 520
    AFA (Acriflavin Feulgen SITSA) 355-425 460
    Alizarin Complexon 530-560 580
    Alizarin Red 530-560 580
    Allophycocyanin 650 661
    ACMA 430 474
    Aminoactinomycin D 555 655
    Aminocoumarin 350 445
    Anthroyl Stearate 361-381 446
    Astrazon Brilliant Red 4G 500 585
    Astrazon Orange R 470 540
    Astrazon Red 6B 520 595
    Astrazon Yellow 7 GLL 450 480
    Atabrine 436 490
    Auramine 460 550
    Aurophosphine 450-490 515
    Aurophosphine G Continue reading “Fluorochromes – Excitation and Emission Wavelengths” »
  • Fluorescent Microspheres – Properties and Applications

    Fluorescent microspheres are round spherical particles that emit bright colors when illuminated by UV light. Ability to emit intense color under UV (black light) illumination provides contrast and visibility of microspheres relative to background materials. In addition to the benefits of conventional high quality microspheres, such as sphericity, smoothness and spreadability among others, fluorescent spheres offer extra sensitivity and detectability for analytical methods. For example, fluorescent microbeads are often used as traces to simulate spread of viruses in medical research.

    Typical applications of fluorescent spheres include: testing of filtration media and systems, vial and container cleaning studies, flow tracing, flow visualization, and fluid mechanics studies, medical imaging and flow cytometry, fluorescence microscopy and photography, as well as biomedical technology research, qualification and validation of medical devices, biomedical diagnostics, process troubleshooting and process flow among others. Specifically fluorescent microspheres are often used for water- and air-flow testing and bead-based diagnostic applications. New unique applications of fluorescent spheres are being discovered daily.

    75-90um Dual Response - Daylight & 365nm UV  20um-1000umFluorescent spheres have a unique ability to appear translucent (clear) and practically invisible under ordinary light, and emit intense visible color when energized by ultraviolet (UV) light. This effect allows scientists and engineers to design blind tests and controlled experiments (e.g. simulate spread of viruses) where the microspheres are invisible to the operator doing the procedure until after the fact, therefore, eliminating any operator bias and uncertainty in the validity of experiment. This unique feature of fluorescent microspheres has numerous applications in biomedical research and process troubleshooting. Continue reading “Fluorescent Microspheres – Properties and Applications” »