Everything about Microspheres
RSS feed
  • Fluorochromes – Excitation and Emission Wavelengths

    Posted on April 28th, 2010 Microsphere Expert

    Below is a very useful table of various Fluorochromes that can be used in research requiring precise responses.  For more information about Fluorescent Microspheres that utilize fluorochromes check out this related article. “Fluorescent Microspheres – Properties and Applications

    The fluorochrome table is also available for download as a pdf.

    Table of Excitation and Emission WavelengthsFLUOROCHROME EXCITATION(nm)
    EMISSION (nm)
    3-Hydroxypyrene 5,8,10-Tri Sulfonic acid 403 513
    5-Hydroxy Tryptamine 380-415 520-530
    5-Hydroxy Tryptamine (5-HT) 400 530
    Acid Fuchsin 540 630
    Acridine Orange (bound to DNA) 502 526
    Acridine Red 455-600 560-680
    Acridine Yellow 470 550
    Acriflavin 436 520
    AFA (Acriflavin Feulgen SITSA) 355-425 460
    Alizarin Complexon 530-560 580
    Alizarin Red 530-560 580
    Allophycocyanin 650 661
    ACMA 430 474
    Aminoactinomycin D 555 655
    Aminocoumarin 350 445
    Anthroyl Stearate 361-381 446
    Astrazon Brilliant Red 4G 500 585
    Astrazon Orange R 470 540
    Astrazon Red 6B 520 595
    Astrazon Yellow 7 GLL 450 480
    Atabrine 436 490
    Auramine 460 550
    Aurophosphine 450-490 515
    Aurophosphine G 450 580
    BAO 9 (Bisaminophenyloxadiazole) 365 395
    BCECF 505 530
    Berberine Sulphate 430 550
    Bisbenzamide 360 600-610
    BOBO 1 462 481
    Blancophor FFG Solution 390 470
    Blancophor SV 370 435
    Bodipy Fl 503 512
    BOPRO 1 462 481
    Brilliant Sulphoflavin FF 430 520
    Calcien Blue 370 435
    Calcium Green 505 532
    Calcofluor RW Solution 370 440
    Calcofluor White 440 500-520
    Calcophor White ABT Solution 380 475
    Calcophor White Standard Solution 365 435
    Cascade Blue 400 425
    Catecholamine 410 470
    Chinacrine 450-490 515
    Coriphosphine O 460 575
    Coumarin-Phalloidin 387 470
    CY3.1 8 554 568
    CY5.1 8 649 666
    CY7 710 805
    Dans (1-Dimethyl Amino Naphaline 5 Sulphonic Acid) 340 525
    Dansa (Diamino Naphtyl Sulphonic Acid) 340-380 430
    Dansyl NH-CH3 in water 340 578
    DAPI 350 470
    Diamino Phenyl Oxydiazole (DAO) 280 460
    Dimethylamino-5-Sulphonic acid 310-370 520
    Diphenyl Brilliant Flavine 7GFF 430 520
    Dopamine 340 490-520
    Eosin 525 545
    Erythrosin ITC 530 558
    Ethidium Bromide 510 595
    Euchrysin 430 540
    FIF (Formaldehyde Induced Fluorescence) 405 435
    Flazo Orange 375-530 612
    Fluorescein Isothiocyanate (FITC) 490 525
    Fluo 3 485 503
    Fura-2 340-380 512
    Genacryl Brilliant Red B 520 590
    Genacryl Brilliant Yellow 10GF 430 485
    Genacryl Pink 3G 470 583
    Genacryl Yellow 5GF 430 475
    Gloxalic Acid 405 460
    Granular Blue 355 425
    Haematoporphyrin 530-560 580
    Hoechst 33258 (bound to DNA) 346 460
    Indo-1 350 405-482
    Intrawhite Cf Liquid 360 430
    Leucophor PAF 370 430
    Leucophor SF 380 465
    Leucophor WS 395 465
    Lissamine Rhodamine B200 (RD200) 575 595
    Lucifer Yellow CH 425 528
    Lucifer Yellow VS 430 535
    Magdala Red 524 600
    Maxilon Brilliant Flavin 10 GFF 450 495
    Maxilon Brilliant Flavin 8 GFF 460 495
    MPS (Methyl Green Pyronine Stilbene) 364 395
    Mithramycin 450 570
    NBD Amine 450 530
    Nile Red 515-530 525-605
    Nitrobenzoxadidole 460-470 510-650
    Noradrenaline 340 490-520
    Nuclear Fast Red 289-530 580
    Nuclear Yellow 365 495
    Nylosan Brilliant Flavin E8G 460 510
    Pararosaniline (Feulgen) 570 625
    Phorwite AR Solution 360 430
    Phorwite BKL 370 430
    Phorwite Rev 380 430
    Phorwite RPA 375 430
    Phosphine 3R 465 565
    Phycoerythrin R 480-565 578
    Pontochrome Blue Black 535-553 605
    Primuline 410 550
    Procion Yellow 470 600
    Propidium Iodide 536 617
    Pyronine 410 540
    Pyronine B 540-590 560-650
    Pyrozal Brilliant Flavin 7GF 365 495
    Quinacrine Mustard 423 503
    Rhodamine 123 511 534
    Rhodamine 5 GLD 470 565
    Rhodamine 6G 526 555
    Rhodamine B 540 625
    Rhodamine B 200 523-557 595
    Rhodamine B Extra 550 605
    Rhodamine BB 540 580
    Rhodamine BG 540 572
    Rhodamine WT 530 555
    Rose Bengal 540 550-600
    Serotonin 365 520-540
    Sevron Brilliant Red 2B 520 595
    Sevron Brilliant Red 4G 500 583
    Sevron Brilliant Red B 530 590
    Sevron Orange 440 530
    Sevron Yellow L 430 490
    SITS (Primuline) 395-425 450
    SITS (Stilbene Isothiosulphonic acid) 365 460
    Stilbene 335 440
    Snarf 1 563 639
    sulphO Rhodamine B Can C 520 595
    Sulpho Rhodamine G Extra 470 570
    Tetracycline 390 560
    TRITC (Tetramethyl Rhodamine Isothiocyanate) 557 576
    Texas Red 596 615
    Thiazine Red R 510 580
    Thioflavin S 430 550
    Thioflavin TCN 350 460
    Thioflavin 5 430 550
    Thiolyte 370-385 477-484
    Thiozol Orange 453 480
    Tinopol CBS 390 4.3
    TOTO 1 514 533
    TOTO 3 642 661
    True Blue 365 420-430
    Ultralite 656 678
    Uranine B 420 520
    Uvitex SFC 365 435
    Xylene Orange 546 580
    XRITC 582 601
    YO PRO 1 491 509

    Fluorescent Microspheres – Properties and Applications

Comments are closed.